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polyclonal rabbit anti phospho p44 42 mapk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc polyclonal rabbit anti phospho p44 42 mapk
    Polyclonal Rabbit Anti Phospho P44 42 Mapk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 99/100, based on 16774 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/polyclonal+rabbit+anti+phospho+p44+42+mapk/Phospho-p44%2F42+MAPK+(Erk1%2F2)+(Thr202%2FTyr204)+Antibody/10__7554_slash_elife__104453__3-290-147-158
    Average 99 stars, based on 16774 article reviews
    polyclonal rabbit anti phospho p44 42 mapk - by Bioz Stars, 2026-09
    99/100 stars

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    Incubation:

    Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.104453 16 of 24 spinal cord and brain sections (30 μm) were incubated in blocking solution (3% normal goat serum [#S- 1000; Vector Laboratories] or normal donkey serum [#017- 000- 121; Jackson ImmunoResearch]) for 2 hr at room temperature and then incubated for 48 hr at 4°C with primary antibodies: polyclonal rabbit anti- tyrosine hydroxylase (TH; 1:1000; #AB152; Millipore); polyclonal sheep anti- TH (1:1000; #AB1542; Millipore); monoclonal mouse anti- noradrenaline transporter (NET; 1:2000; #NET05- 2; Mab Technologies); polyclonal rabbit anti- green fluorescent protein (GFP; 1:1000; #598; MBL International); monoclonal rabbit anti- hemagglutinin (HA)- tag (1:1000; #3724; Cell Signaling); monoclonal rat antiglial fibrillary acidic protein (GFAP; 1:2000; #13- 0300; Invitrogen); polyclonal goat anti- SRY- related high- mobility group box 9 (SOX9; 1:1000; # AF3075; R&D Systems); polyclonal goat anti- paired box 2 (PAX2; 1:500; # AF3364; R&D Systems); monoclonal rat anti- mCherry (1:2000; #M11217; Thermo Fisher Scientific); polyclonal rabbit anti- phospho- p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (pERK; 1:500; #9101; Cell Signaling); anti- isolectin B4 (IB4)- biotin conjugate (1:1000; # I21414; Thermo Fisher Scientific); and monoclonal rabbit anti- c- FOS (1:5000; #226 008; Synaptic systems). .. After incubation, tissue sections were washed and incubated for 3 hr at room temperature with secondary antibodies (Alexa Fluor 488, 546 and/or 555; #A11001, A11008, A11035, A11056, A11081, S32351; Thermo Fisher Scientific: #ab150178; Abcam: DyLight 405; #705- 475- 147; Jackson ImmunoResearch).

    Article Title: The prostacyclin agonist iloprost aggravates fibrosis and enhances viral replication in enteroviral myocarditis by modulation of ERK signaling and increase of iNOS expression.
    Article Snippet: Enteroviruses, such as coxsackieviruses of group B (CVB), are able to induce a chronic inflammation of the myocardium, which may finally lead to the loss of functional tissue, remodeling processes and the development of fibrosis, thus affecting the proper contractile function of the heart.. In other fibrotic diseases like scleroderma, the prostacyclin agonist iloprost was found to inhibit the extracellular signal-regulated kinase (ERK, p44/ 42 MAPK), a mitogen-activated protein kinase, and consecutively, the expression of the profibrotic cytokine connective tissue growth factor (CTGF), thereby preventing the development of fibrosis.. As CTGF was found to mediate fibrosis in chronic CVB3 myocarditis as well, we evaluated whether the in vivo application of iloprost is capable to reduce the development of ERK/CTGF-mediated fibrosis in enteroviral myocarditis.

    Blocking Assay:

    Article Title: Descending locus coeruleus noradrenergic signaling to spinal astrocyte subset is required for stress-induced mechanical pain hypersensitivity
    Article Snippet: .. DOI: https://doi.org/10.7554/eLife.104453 16 of 24 spinal cord and brain sections (30 μm) were incubated in blocking solution (3% normal goat serum [#S- 1000; Vector Laboratories] or normal donkey serum [#017- 000- 121; Jackson ImmunoResearch]) for 2 hr at room temperature and then incubated for 48 hr at 4°C with primary antibodies: polyclonal rabbit anti- tyrosine hydroxylase (TH; 1:1000; #AB152; Millipore); polyclonal sheep anti- TH (1:1000; #AB1542; Millipore); monoclonal mouse anti- noradrenaline transporter (NET; 1:2000; #NET05- 2; Mab Technologies); polyclonal rabbit anti- green fluorescent protein (GFP; 1:1000; #598; MBL International); monoclonal rabbit anti- hemagglutinin (HA)- tag (1:1000; #3724; Cell Signaling); monoclonal rat antiglial fibrillary acidic protein (GFAP; 1:2000; #13- 0300; Invitrogen); polyclonal goat anti- SRY- related high- mobility group box 9 (SOX9; 1:1000; # AF3075; R&D Systems); polyclonal goat anti- paired box 2 (PAX2; 1:500; # AF3364; R&D Systems); monoclonal rat anti- mCherry (1:2000; #M11217; Thermo Fisher Scientific); polyclonal rabbit anti- phospho- p44/42 MAPK (ERK1/2) (Thr202/Tyr204) (pERK; 1:500; #9101; Cell Signaling); anti- isolectin B4 (IB4)- biotin conjugate (1:1000; # I21414; Thermo Fisher Scientific); and monoclonal rabbit anti- c- FOS (1:5000; #226 008; Synaptic systems). .. After incubation, tissue sections were washed and incubated for 3 hr at room temperature with secondary antibodies (Alexa Fluor 488, 546 and/or 555; #A11001, A11008, A11035, A11056, A11081, S32351; Thermo Fisher Scientific: #ab150178; Abcam: DyLight 405; #705- 475- 147; Jackson ImmunoResearch).



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